November 24, 2014

Scientists seek ways to clone preserved mammoth

Kenison Garratt 
Staff Writer


A well preserved woolly mammoth, named Buttercup, found in Siberia in May of 2013 is being analyzed by scientists to find out if it has enough DNA to be cloned. Buttercup had been well preserved beneath the permafrost, with three legs, most of her body and head, and her trunk remaining intact. In the three days following her discovery, scientists focused on finding out more about her life before they had to freeze her again to prevent rot.

Buttercup lived approximately 40,000 years ago. She often ate dandelions and buttercups, she had eight calves and lost one baby, and died in her mid-50s. It is believed that the woolly mammoth became trapped in a bog and was attacked, most likely by wolves, and eaten alive. When discovered, she oozed a red liquid; the scientists present assumed it to be blood. Dr. Tori Herridge, a paleobiologist at London’s Natural History Museum, says, “As a shorthand, people call it blood, but in reality what you have is a reddish fluid. The researchers didn’t find any intact red blood cells at all, but the red still came from the hemoglobin.”


Russian and Korean scientists have been looking for a full strand of DNA to use to clone Buttercup. Insung Hwang, a geneticist at the South Korean biotech company Sooam says, “There is the possibility of finding something that is amazing. We are very hopeful that this mammoth can give us an accurate genomic map that we can use as a template in the future to possibly bring back the mammoth.” Mammoths found previously only had a little bit of blood and no complete DNA strands. So far, no complete genome has been found in Buttercup, either. If scientists cannot find a complete strand, there are other methods to bring back the mammoth.

Scientists may be able to take some long fragments of DNA from tissue and put them together to form a complete genome. Also, if unable to find a full, undamaged genome, Harvard University Researcher George Church has another idea: To combine Buttercup’s DNA with that of an elephant. He wants to take specific traits from the mammoth, such as resistance to cold, the hair and the extra fat, and add these to the Asian elephant, which is the closest relative of the woolly mammoth.

Herridge finds the cloning process to be an ethical conflict. An elephant would have to be used as a surrogate, carrying the mammoth for 22 months. During this period of time, the elephant could be damaged and afterwards, the mammoth may not even survive. “Cloning a mammoth will require you to experiment on probably many, many Asian elephants,” Herridge says. The new born would have to adapt to the new environment, live in captivity and likely be doomed to a lonely existence. Woolly mammoths were allegedly social creatures, and this modern day mammoth would find itself on its own.

Sir Ian Wilmut, the man behind the cloning of Dolly the Sheep, believes it could be worthwhile. “I think it should be done as long as we can provide great care for the animal. If there are reasonable prospects of them being healthy, we should do it. We can learn a lot about them,” Wilmut says.

The amount of time before the woolly mammoth walks the Earth again is unclear.

Last month, the Russian Website Yakutia Today said the revival project could take until 2045. However, a new documentary, “How to Clone a Woolly Mammoth” that includes details from the autopsy airing on the Smithsonian Channel at 8 p.m. on Nov. 29, is less specific on the time frame. It is possible that the mammoth could be brought back sooner. Hwang says, “We’re trying hard to make this possible within our generation.”